Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC205398 . Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 44.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Protein Data

RefSeq: NP_659483	RefSeq Size: 1288	RefSeq ORF: 1155
LocusID: 125972	Cytogenetic: 19p13.11
Synonyms : CMH19; CRT2; CT93
Summary: The protein encoded by this gene belongs to the calreticulin family, members of which are calcium-binding chaperones localized mainly in the endoplasmic reticulum. This protein is also localized to the endoplasmic reticulum lumen, however, its capacity for calcium-binding may be absent or much lower than other family members. This gene is specifically expressed in the testis, and may be required for sperm fertility. Mutation in this gene has been associated with familial hypertrophic cardiomyopathy. [provided by RefSeq, Dec 2011].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens calreticulin 3 (CALR3) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens calreticulin 3 (CALR3) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens calreticulin 3 (CALR3) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of calreticulin 3 (CALR3) Validated Antibodies • Purified CALR3 (Calreticulin 3) mouse monoclonal antibody, Clone 1D2 • Purified CALR3 mouse monoclonal antibody,clone 1D1 • Purified CALR3 (Calreticulin 3) mouse monoclonal antibody, Clone Clone 1E1 • Purified CALR3 (Calreticulin 3) mouse monoclonal antibody, Clone 1G4 • Carrier-free (BSA/glycerol-free) CALR3 mouse monoclonal antibody,clone 1E1 • Carrier-free (BSA/glycerol-free) CALR3 mouse monoclonal antibody,clone 1D1 • Carrier-free (BSA/glycerol-free) CALR3 mouse monoclonal antibody,clone 1D2 • Carrier-free (BSA/glycerol-free) CALR3 mouse monoclonal antibody,clone 1G4 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CALR3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CALR3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Calr3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CALR3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Calr3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CALR3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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