Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC219125 . Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 33.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families:

Protein Data

RefSeq: NP_853628	RefSeq Size: 1857	RefSeq ORF: 933
LocusID: 7378	Cytogenetic: 7p12.3
Synonyms : UDRPASE; UP; UPASE; UPP
Summary: The 2 known types of pyrimidine nucleoside phosphorylases, uridine phosphorylase (UP; EC 2.4.2.3) and thymidine phosphorylase (TP; EC 2.4.2.4), in the presence of orthophosphate, catalyze the reversible phosphorolysis of uridine and thymidine or deoxyuridine, respectively, to free bases and ribose-1-phosphate or deoxyribose-1-phosphate. Pyrimidine nucleoside phosphorylases can add ribose or deoxyribose to pyrimidine bases to form nucleosides that can be incorporated into RNA or DNA (Watanabe and Uchida, 1995 [PubMed 7488099]).[supplied by OMIM, Aug 2009].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens uridine phosphorylase 1 (UPP1), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens uridine phosphorylase 1 (UPP1), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens uridine phosphorylase 1 (UPP1), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of uridine phosphorylase 1 (UPP1), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • UPP1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • UPP1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Upp1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • UPP1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Upp1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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