LSBio offers more than 3,500 IHC-plus™ antibodies that have been extensively tested in immunohistochemistry (IHC) against formalin-fixed paraffin-embedded (FFPE) human tissues.
LSBio's IHC-plus™ antibodies have been tested and identified as being optimal for use in immunohistochemistry (IHC) against formalin-fixed paraffin-embedded (FFPE) human tissues under
LSBio's standardized IHC-plus™ protocol.
Each antibody has been characterized at four different concentrations against 21 normal human tissues, plus additional relevant controls.
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In order to be selected as an IHC-plus™ brand antibody, antibodies must have a close correlation to the published literature, be high affinity, display minimal staining artifacts, and have a high signal-to-noise ratio, such that its specific staining is considerably higher than its level of nonspecific background staining.
Only the best antibodies receive approval and are given the designation as LSBio's premier IHC-plus™ antibodies.
For Immunohistochemistry (IHC) Validation, LSBio has tested and validated monoclonal antibodies (mouse, rabbit, rat), polyclonal antibodies (rabbit, goat, sheep, llama, chicken), and human single and double chain antibodies for immunohistochemistry. The following summarizes our methods and approach toward immunohistochemistry (IHC) antibody validation:
- Step 1 : Choosing the Antibody
- Step 2 : Determining Antibody Specificity
- Step 3 : Tissue Fixation for IHC
- Step 4 : Tissue Validation
- Step 5 : IHC Validation and Pathology Analysis
- Step 6 : Troubleshooting
Human Placenta (formalin-fixed, paraffin-embedded) stained with SDC1 antibody LS-B3341 at 15 ug/ml followed by biotinylated
goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
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