Mycoplasma : Detection and Treatment of Contamination

Do you suspect mycoplasma contamination?

Ready-to-use PCR Mix for the detection of mycoplasma in cell culture
EZ-PCR Mycoplasma Test Kit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells.

The primer set allows detection of various mycoplasma species (M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum), as well as Acholeplasma and Spiroplasma species, with high sensitivity and specificity.

Advantages
- Highly sensitive
- Mycoplasma-specific primers with broad range
- Convenient and user-friendly: supplied with complete reaction mix
- Requires no more than 10-20 minutes of actual work
- Samples are easy to prepare
- Results are easily determined with a single PCR process
- Rapid: results obtained in no more than 5 hours
- No need for internal control application steps
- No loading dye needed for the agarose gel

Product Name	Cat#	Size
EZ-PCR Mycoplasma test kit	20-700-10	10 tests
EZ-PCR Mycoplasma test kit	20-700-20	20 tests

You already know mycoplasma has found its way into your cell culture ?

Biological Industries offers the BIOMYC-1, BIOMYC-2 and BIOMYC-3 antibiotic combinations, which have been shown to be effective in the elimination of mycoplasma species that account for 90% of the contaminations found in cell cultures. When using according to instructions, no cytotoxic effects will occur.

BIOMYC-1 and BIOMYC-2
BIOMYC-1 is a solution based on the antibiotic Tiamutin. BIOMYC-2 is a solution based on the antibiotic Minocycline, which is a member of the Tetracycline group.

These two antibiotics have been shown to be effective in eliminating the mycoplasma species frequently present in contaminated cell cultures. Furthermore, the mycoplasmas do not develop resistance to these antibiotics – a common occurrence with other antibiotic treatment methods.

BIOMYC3
BIOMYC-3 is a solution based on the antibiotic Ciprofloxacin. This antibiotic belongs to the Fluoroquinolone group that inhibits synthesis of the enzyme DNA gyrase, which is responsible for supercoiling in bacterial DNA.

Product Name	Cat#	Size
BIOMYC-1 Antibiotic Solution 100X	03-036-1D	10 ml
BIOMYC-1 Antibiotic Solution 100X	03-036-1C	20 ml
BIOMYC-1 Antibiotic Solution 100X	03-036-1B	100 ml
BIOMYC-2 Antibiotic Solution 100X	03-037-1D	10 ml
BIOMYC-2 Antibiotic Solution 100X	03-037-1C	20 ml
BIOMYC-2 Antibiotic Solution 100X	03-037-1B	100 ml
BIOMYC-3 Antibiotic Solution 100X	03-038-1D	10 ml
BIOMYC-3 Antibiotic Solution 100X	03-038-1C	20 ml
BIOMYC-3 Antibiotic Solution 100X	03-038-1B	100 ml

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