La KAPA HiFi DNA Polymerase offre une amplification de librairie haute fidélité pour le NGS avec un haut rendement, faible biais et la meilleure uniformité de la couverture de séquence.
These characteristics are retained in KAPA HiFi Uracil+ DNA Polymerase, a modified version of KAPA HiFi engineered to tolerate uracil residues. The new enzyme is particularly well suited for applications employing bisulfite DNA conversion, which typically produces low concentrations of AT-rich DNA.
Applications:
DNA Methylation analysis - amplification of bisulfite-converted DNA for sequencing
Amplification of damaged DNA samples
Prevention of false-positive results due to carryover amplicon contamination
Catalog #
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Description
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Size
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KK2801
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KAPA HiFi HotStart Uracil+ ReadyMix
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50 x 50 µL reactions |
KK2802
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KAPA HiFi HotStart Uracil+ ReadyMix |
250 x 50 µL reactions |

Human whole genome bisulfite-treated libraries were amplified using standard protocols with either 12, 14 or 16 cycles, and the amplified libraries analyzed using a Bioanalyzer 2100 High Sensitivity DNA chip.
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