KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency and sensitivity.
KAPA Taq and KAPA Taq HotStart DNA Polymerase is ideally suited for:
- High throughput PCR
- Amplification of low copy DNA templates
- Multiplex PCR
- Specific amplification of complex templates
KAPA Taq and KAPA Taq HotStart PCR Kits are supplied with novel buffers designed for optimal enzyme activity and performance. The proprietary formulation facilitates specific primer annealing, which translates to higher yields of specific product when compared to traditional Taq buffers.
The 5x buffer KAPA Taq HotStart Buffer is supplied without MgCl2 for optimal flexibility. KAPA Taq HotStart DNA Polymerase may, however, be used in combination with any standard Taq buffer with a pH of 8.3 or higher.
KAPA Taq and KAPA Taq HotStart DNA Polymerase has 5’→3’ polymerase and 5’→3’ exonuclease activities, but no 3’→5’ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. PCR products generated with KAPA Taq and KAPA Taq HotStart are A-tailed and may be cloned into TA cloning vectors.
KAPA Taq HotStart outperforms the competition
A 500 bp fragment of the CCR5 gene was amplified using 100 ng, 10 ng, 1 ng or 100 pg of human genomic DNA as template. KAPA Taq HotStart exhibits improved sensitivity, specificity, and yield when compared with competitive hotstart products. All reactions were performed using the manufacturer recommended protocols.
KAPA Taq HotStart improves the specificity and sensitivity in PCR
A 270 bp amplicon was amplified from mycoplasma DNA with KAPATaq or KAPATaq HotStart. Sensitivity was tested using a 10x template dilution series starting with 1 ng of DNA. Higher sensitivity and less primer dimers are observed when using KAPA Taq HotStart.
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