We propose to clone your cDNA in different vectors.
This cloning will allow you to perform :
- your Protein production, after transfection in a cell line
- your Antibodies production, after immunization of animals
Standard protocol of cDNA cloning :
- Design and production of probes
- Performing of the RT-PCR
- Cloning of the obtained cDNA
- Checking the cloning by DNA sequencing and/or enzymatic restriction