The perfect RNAi knockdown

RNA Interference With 29-mer shRNA (HuSH-29)
More than 37 000 available !

29mer up to 100 times more efficient than 21mer

Superior tools for RNAi

Purified plasmid at no extra cost

All HuSH constructs are fully sequenced

All our products include top-notch technical service

Citations in peer-reviewed journals

More information

The kit includes : 4 unique gene-specific shRNA plasmids and two negative control plasmids

Search for your shRNA on our website.
www.clinisciences.com


293T cells were cotransfected with pRS vector or shRNA constructs against various regions of EGFP together with an EGFP expression plasmid in 1:1 molar ratio using Fugene reagent. Two days post-transfection, pictures were taken using a fluorescent microscope. Significant downregulation of EGFP expression was detectable in 4 out of 5 constructs.

References for HuSH shRNA

Tumor Suppressor Activity of CCAAT/Enhancer Binding Protein Alpha Is Epigenetically Down-regulated in Head and Neck Squamous Cell Carcinoma Kristi L. Bennett, Björn Hackanson, Laura T. Smith, Carl D. Morrison, James C. Lang, David E. Schuller, Frank Weber, Charis Eng and Christoph Plass
Cancer Res., May 2007; 67: 4657

L-arginine availability regulates T-lymphocyte cell-cycle progression
Paulo C. Rodriguez, David G. Quiceno, and Augusto C. Ochoa
Blood, Feb 2007; 109: 1568 - 1573.

Naked2 Acts as a Cargo Recognition and Targeting Protein to Ensure Proper Delivery and Fusion of TGF-a containing Exocytic Vesicles at the Lower Lateral Membrane of Polarized MDCK Cells
Cunxi Li, Mingming Hao, Zheng Cao, Wei Ding, Ramona Graves-Deal, Jianyong Hu, David W. Piston, and Robert J. Coffey
Mol. Biol. Cell, Jun 2007; 10.1091/mbc.E07-02-0172.

References for shRNA

Mammalian RNAi: a practical guide. P. Sandy, A. Ventura, T. Jacks, Biotechniques. 2005 August, 39, 215-224

Synthetic dsRNA Dicer substrate enhance RNA potency and efficiency.  Dong-Ho Kim, Mark A Behlke, Scott D Rose, Mi-Sook Chang, Sangdun Choi & John J Rossi. Nat. Biotechnol. 2004 Dec 26. 23, 222-226

Synthetic shRNAs as potent RNAi triggers. Despina Siolas, Cara Lerner, Julja Burchard, Wei Ge, Peter S Linsley, Patrick J Paddison, Gregory J Hannon & Michele A Cleary.  Nat.Biotechnol. 2004 Dec 26. 23, 227-231

Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. Richard J Fish and Egbert KO Kruithof BMC Molecular Biology 2004, 5:9

RNAi-Mediated Silencing of Insulin Receptor Substrate1 (IRS-1) Enhances Tamoxifen-Induced Cell Death inMCF-7 Breast Cancer Cells Gregory Cesarone, Cecilia Garofalo, Marc T. Abrams, Olga Igoucheva, Vitali Alexeev, Kyonggeun Yoon, Eva Surmacz, Eric Wickstrom  J Cell Biochem. 2006 May 15;98(2):440-50

Small non-coding RNAs as magic bullets Fritz Eckstein. Trends in Biochemical Sciences, 2005 Aug, 30:8, 445-452.

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