Molecular biology tools

Vector Laboratories' reagents for labeling, detection and manipulation of nucleic acids offer sensitivity, reliability, simplicity and economy.

Demandez votre brochure "A guide to nucleic acid labeling and detection systems"
End labeling of oligonucleotides :
3' EndTag DNA Labeling System The 3' EndTag Nucleic Acid Labeling System enables simple and uniform labeling of DNA 3' ends. A choice of diverse labels (fluorochromes, haptens, affinity ligands) gives this system outstanding versatility. No interference with hybridization or sequence-specific protein binding More efficient labeling for short oligonucleotides More economical Cat# : MB-9002 Thiol-reactive labeling reagents Zoom	5' EndTag Nucleic Acid Labeling System The 5' EndTag™ Nucleic Acid Labeling System facilitates the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the 5' end of unmodified oligonucleotides or 5'-OH modified DNA or RNA. Ideal for : Labeling PCR and sequencing primers Binding of capture probes to affinity matrices Gel shift assays Cat# : MB-9001 Thiol-reactive labeling reagents Zoom
Multiple site labeling of nucleic acid :
PHOTOPROBE® Amine PHOTOPROBE® Amine : incorporates primary amines into nucleic acids. PHOTOPROBE® (Long Arm) Biotin : has extra long linker arm and should be used if increased distance between the sample and the tag is needed. PHOTOPROBE® Biotin : incorporates biotin in one reaction step. PHOTOPROBE® Biotin Labeling/Detection Kit	FastTag Basic Labeling Kit Incorporation of a variety of labels at multiple sites along the entire length of the nucleic acid. Label single- or double-stranded DNA, RNA or PNA (peptide nucleic acid) : incorporate on average one label every twenty base pairs. System based on aryl azide chemistry in which the reagent, when exposed either to heat or light, becomes activated and incorporates into the nucleic acid to which any thiol-reactive compound can be attached. Labeling is not base-specific. Cat# : MB-8000 Zoom
Detection of nucleic acid label :
Label detection All antibodies to the labels are affinity-purified and are ideal for detecting labeled probes in such applications as in situ hybridization or northern and Southern blotting. Antibodies and reagents for the detection of the following labels : Biotin DNP (Dinitrophenyl) Fluorescein Fucose Texas Red®	Complementary Detection Reagents Fluorescent systems Ideal for applications such as fluorescence in situ hybridization. Biotinylated anti-avidin and biotinylated anti-steptavidin are effectively used for amplifying fluorescent signals in biotin-avidin/streptavidin systems. Vectashield® Mounting Media Anti-fade and anti-photobleaching properties : unsurpassed in preventing the rapid loss of fluorescence during microscopic examination. Chromogenic substrates for enzyme-based detection DuoLuX™ Chemiluminescent/Fluorescent substrates Permanent Mounting Medium for Chromogenic substrates
Other molecular biology products from Vector Laboratories :
Northern and Southern blot hybridization HYBEX™ Hybridization Solution The hybridization of nucleic acid probe to the target on the blot is simplified using this ready-to-use solution which contains no formamaide and can be used with both nylon and nitrocellulose membranes. UltraSNAP™ Detection Kit For the detection of biotinylated nucleic acids on nylon or nitrocellulose blots. DNA Molecular Weight Markers Animal-Free Blocker™ (5x)	Optimization of probe length for ISH NicKit™ p.s.o. (probe size optimization) has been developed to reduce the size of large DNA probes using a photo/enzymatic procedure without the problem of over- or under- digestion experienced with other methods. A smaller probe size generally results in better signal and lower background for ISH applications, probably due to better tissue penetration of the probe. Cat# : MB-1905
Affinity binding Genomic/cDNA substraction, microsatellite isolation nd library screening by hybrid capture require the removal or immobilization of nucleic acid hybrids. Affinity bonding matrices are available for use with PHOTOPROBE®-, FastTag®-, or EndTag™- labeled probes for such applications. Irreversible binding matrix : VECTREX® Avidin D Reversible binding matrices : VECTREX® Avidin DLA VECTREX® Avidin DLA Binding and Elution Kit VECTREX® Avidin AAL VECTREX® Avidin AAL Binding and Elution Kit	Seperate Same-Size DNA by Sequence Variation The ability to resolve DNAs of similar size in a gel is critical for applications such as differential display and Rapid Amplification of Polymorphic DNAs (RAPD) in which multiple DNA species of similar size need to be separated before subsequent excision and analysis. Two sequence specific DNA lignads in the Resolve-It™ kit bind to GC- or AT- rich sites on DNA in a sequence specific manner. Cat# : MB-1401

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