The most efficient method to purify polyclonal antibodies with a peptid-coated column.
It allows to obtain highly-specific antibodies inducing a very low noise ratio.
Purification on protein G-affinity column allows purification of any kind of monoclonals and polyclonals.
The yield is around 90% of the serum IgG fraction from culture supernatant or ascites.
Purification on protein A-affinity column is similar to protein G-affinity column with a yield around 90% of IgG fraction.
Choice between A and G-affinity columns depends on antibody isotype or host specie.