Immunoprecipitation

Selective enrichment of a chromatin fraction containing a specific target.

RIP-chip works on the same principle as the widely used ChIP-chip. It immunoprecipitates the ribonucleoprotein (RNP) from the cell extracts using an antibody raised against the RBP of interest. This simple procedure is then followed by microarray analysis. While microarrays determine the sequences of the RNA targets by hybridization, direct sequencing approaches (RIP-seq) can also be used to reveal RNA targets of RBPs.