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> 7α-Hydroxycholesterol [566-26-7]

7α-Hydroxycholesterol [566-26-7]

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Référence HY-N7264-5mg

Conditionnement : 5mg

Marque : MedChemExpress


Description

7α-Hydroxycholesterol is a cholesterol oxide and can serve as a biomarker for oxidative stress and lipid peroxidation. 7α-Hydroxycholesterol has cytotoxic and pro-inflammatory activities. 7α-Hydroxycholesterol can also inhibit sterol synthesis and reduce the activity of HMG-CoA reductase. 7α-Hydroxycholesterol can be used in the research of diseases such as diabetes and atherosclerosis[1][2][3][4][5].

Cellular Effect
Cell Line Type Value Description References
C6 IC50
40 μM
Compound: 4, 7alpha-OHC
Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method
Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method
[PMID: 24211631]
HT-29 IC50
15.5 μM
Compound: 5
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
[PMID: 19473028]
HT-29 IC50
15.5 μM
Compound: 5
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HT-29 IC50
15.5 μM
Compound: 7
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
In Vitro

7α-Hydroxycholesterol (2.5-160 μM; 24 h) has cytotoxicity against 158N and C6 cells, with IC50 values of approximately 15 μM and 40 μM, respectively[1].
7α-Hydroxycholesterol (8 μg/mL; 2-4 h) significantly reduces the sterol synthesis rate and HMG-CoA reductase activity in primary hepatocytes or L-cell cultures[2].
7α-Hydroxycholesterol (5 µg/mL; 48 h) increases the transcriptional level of TLR6, promotes the expression of CD14, and induces monocytes to produce chemokines such as CCL2, CCL3, and CCL4 in monocytes/macrophages. The mechanism involves the phosphorylation of Akt, Src, ERK1/2, and the p65 subunit[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7α-Hydroxycholesterol Related Antibodies

Real Time qPCR[3]

Cell Line: THP-1 cells
Concentration: 5 µg/mL
Incubation Time: 48 h
Result: Significantly increased the mRNA levels of TLR6 and CD14.
Masse moléculaire

402.65

Formule

C27H46O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2(02)[C@]3(02)[C@H](O)C=C4C[C@@H](O)CC[C@]4(C)[C@@]3(02)CC[C@]12C
Structure Classification
Initial Source

fish oil, serum
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité
In Vitro: 

DMSO : 50 mg/mL (124.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 10 mg/mL (24.84 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 10 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 10 mg/mL (24.84 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 10 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Pureté et documentation
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