Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken/Turtle Acidic Cytokeratin Antibody [Sodium Azide Free]
Anti-Acidic Cytokeratin Antibody, Sodium Azide Free
Anti-Acidic Cytokeratin Antibody, Sodium Azide Free
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Product Description
Specifications
| Size | 100 µg |
| Species | Human, Mouse, Rat |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P13645 |
| Gene Id | 3858 |
| Gene Symbols | KRT10, KPP |
| Protein Name / Synonyms | Keratin, type I cytoskeletal 10 (Cytokeratin-10) (CK-10) (Keratin-10) (K10) |
| Preparation | Solubilized keratin extract from human stratum corneum |
| Isotype | IgG1 |
| Purity | Protein G purified |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | FACS: 0.5-1ug/million cells IF: 1-2ug/ml Western Blotting: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Cytokeratins, also called keratins or simply CKs, are a family of fibrous structural proteins and a major structural component in the outer layer of human skin, as well as hair and nails. There are 20 human epithelial keratins which can be divided into two subfamiles: acidic and basic (more recent human genone sequencing has identified an additional 20 members). The formation of cytokeratin intermediate filaments requires the pairing of at least one acidic and one basic subfamily member. Members of the same keratin subfamily share extended sequence homology while members of different subfamilies show only limited sequence homology.
This antibody is specific for the 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), and 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Many studies have shown the usefulness of CKs as markers in cancer research and tumor diagnosis, as epithelial tumors generally maintain the same CK expression patterns as their counterpart normal tissue. Clone AE1 is commonly used with clone AE3 as a pan cytokeratin antibody cocktail (Cat No V2330).
Storage
Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.