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> Anti-AGT Antibody (1Z261)

Anti-AGT Antibody (1Z261)

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Référence NB-64-115558-100uL

Conditionnement : 100uL

Marque : Neo Biotech


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Anti-AGT Antibody (1Z261)

(Synonyms: Serpin A8, Angiotensinogen) Copy Product Info

Synonyms: Serpin A8, Angiotensinogen

Catalog No. TMAC-00208 Copy Product Info
Anti-AGT Antibody (1Z261) is a Rabbit antibody targeting AGT. Anti-AGT Antibody (1Z261) can be used in ICC/IF,IP,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Description
Anti-AGT Antibody (1Z261) is a Rabbit antibody targeting AGT. Anti-AGT Antibody (1Z261) can be used in ICC/IF,IP,WB.
SynonymsSerpin A8, Angiotensinogen
Ig Type
IgG
Clone
1Z261
Reactivity
Human
Verified Activity
1. ICC staining Angiotensinogen in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
2. ICC staining Angiotensinogen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Angiotensinogen in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Angiotensinogen in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Anti-AGT Antibody (1Z261),Anti-AGT Antibody (1Z261),Anti-AGT Antibody (1Z261),Anti-AGT Antibody (1Z261)
Application
ICC/IF
IP
WB
Recommended Dose
WB: 1:1000; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundAngiotensin is formed from a precursor, angiotensinogen, which is produced by the liver and found in the a-globulin fraction of plasma. The lowering of blood pressure is a stimulus to secretion of Renin by the kidney into the blood. Renin cleaves from angiotensinogen a terminal decapeptide, Angiotensin I (Ang I). This is further altered by the enzymatic removal of a dipeptide to form Angiotensin II (Ang II). Screening a panel of human-mouse somatic cell hybrids confirmed the assignment of the AGT locus to human chromosome 1. Angiotensin, an octapeptide hormone, is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. It has been shown that mechanical stress causes release of Angiotensin from cardiac myocytes and that Angiotensin acts as an initial mediator of the hypertrophic response. Angiotensin treatment also stimulates phosphorylation of Shc, FAK and MAP kinases and induces MKP-1, indicating stimulation of growth factor pathways. Angiotensin stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by co-immunoprecipitation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
P01019
Chemical Properties
Molecular WeightTheoretical: 53 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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