Anti-GATA3 Antibody (1N923)
Référence NB-64-120170-100uL
Conditionnement : 100uL
Marque : Neo Biotech
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Anti-GATA3 Antibody (1N923)
(Synonyms: Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA 3) Copy Product InfoSynonyms: Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA 3
Catalog No. TMAH-00475 Copy Product Info
Anti-GATA3 Antibody (1N923) is an antibody targeting GATA3. Anti-GATA3 Antibody (1N923) can be used in ELISA,IHC,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-GATA3 Antibody (1N923) is an antibody targeting GATA3. Anti-GATA3 Antibody (1N923) can be used in ELISA,IHC,WB. |
| Synonyms | Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA 3 |
| Ig Type | Rabbit IgG |
| Clone | 1N923 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: RAW264.7 whole cell lysate -All lanes: GATA3 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 48, 49 kDa -Observed band size: 48 kDa 2. IHC image of TMAH-00475 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00475 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA IHC WB |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | T-cells and endothelial cells. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human GATA3 |
| Antigen Species | Human |
| Gene Name | GATA3 |
| Gene ID | 2625 |
| Uniprot ID | P23771 |
| Biology Area | Epigenetics and Nuclear Signaling, Developmental biology, Immunology, Stem cells |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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