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Anti-GATA4 Antibody (5B64)

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Référence NB-64-156309-50uL

Conditionnement : 50uL

Marque : Neo Biotech


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Anti-GATA4 Antibody (5B64)

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Catalog No. TMAB-06359 Copy Product Info
Anti-GATA4 Antibody (5B64) is a Rabbit antibody targeting GATA4. Anti-GATA4 Antibody (5B64) can be used in WB,ICC/IF,FCM.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-GATA4 Antibody (5B64) is a Rabbit antibody targeting GATA4. Anti-GATA4 Antibody (5B64) can be used in WB,ICC/IF,FCM.
Ig Type
IgG
Clone
5B64
Reactivity
Human,Mouse,Rat
Verified Activity
1. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (GATA4) monoclonal Antibody, unconjugated (TMAB-06359) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with GATA4 monoclonal antibody, unconjugated (TMAB-06359) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
WB
ICC/IF
FCM
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function.In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression.
Antigen Details
Immunogen
A synthesized peptide: human GATA4
Antigen Species
Human
Gene Name
GATA4
Gene ID
2626
Protein Name
Transcription factor GATA-4
Uniprot ID
P43694
Function
Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement.
Chemical Properties
Molecular WeightTheoretical: 45 kDa. Actual: 60 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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