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> Anti-HSP90 alpha/beta Antibody

Anti-HSP90 alpha/beta Antibody

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Référence CQA8111-200ul

Conditionnement : 200ul

Marque : Cohesion Biosciences


  • Western blot analysis of HSP90 alpha/beta expression in rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 90 kD)
  • Immunohistochemical analysis of HSP90 alpha/beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of HSP90 alpha/beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Product Name:Anti-HSP90 alpha/beta Antibody
Cat No:CQA8111
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH, IF/IC, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit monoclonal antibody to HSP90 alpha/beta
Immunogen：Recombinant protein of human Hsp90 beta
Purification：The antibody was purified by immunogen affinity chromatography.
Clonality：Monoclonal
Form：Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
Gene Symbol：HSP90AA1; HSP90AB1
Alternative Names：HSP90AA1; HSP90A; HSPC1; HSPCA; Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Renal carcinoma antigen NY-REN-38; HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84
Entrez Gene (Human)： 3320; 3326;
Entrez Gene (Mouse)： 15519; 15516;
Entrez Gene (Rat)： 299331; 301252;
SwissProt (Human)： P07900; P08238;
SwissProt (Mouse)： P07901; P11499;
SwissProt (Rat)： P82995; P34058;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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