Anti-Human IL-8 (Clone 6217)
Référence I-176-100
Conditionnement : 100µg
Marque : Leinco Technologies
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AntiHuman IL8 [Clone 6217]
AntiHuman IL8 [Clone 6217]
Product No.: I176
Clone 6217 Target IL8 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin8, CXCL8, MDNCE, Neutrophil Activating Factor (NAF), NAP1 Fractalkine MucinLike Stalk, 310C, AMCFI, GCP1, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, AP1, SCYB8, TSG1, BENAP Isotype IgG1 Applications CyTOF® , ELISA Cap , FC , ICC , N , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant IL8/CXCL8 (Accession # P10145) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at 20°C to 70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830570 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 28 µg/ml. A suitable detection antibody is PN:I260 at a concentration of approximately 0.10.4 µg/ml. A suggested standard for this assay is PN:I190. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL8. A suggested method would be to stain cells expressing IL8 with 0.25 µg/106 cells. Western Blotting: To detect Human IL8 this monoclonal antibody can be used at a concentration of 12 µg/ml. This monoclonal antibody should be used in conjunction with compatible secondstep reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL8 is 5 ng/lane under either reducing or nonreducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I190. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IL8 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL8 (at 20 ng/ml) is 0.08 0.4 µg/ml. Immunocytochemistry: Suitable for use at concentration of 825 µg/mL. CyTOFready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse AntiHuman Interleukin8 (IL8) recognizes Human IL8. This monoclonal antibody was purified using multistep affinity chromatography methods such as Protein A or G depending on the species and isotype. Background IL8 is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL8 in their storage vesicles. IL8 is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Gene of IL8 is believed to play a role in the pathogenesis of bronchiolitis. While neutrophil granulocytes are the primary target cells of IL8 there is a relative wide range of cells (endothelial cells, macrophages, mast cells, keratinocytes) responding to this chemokine, too. When first encountering an antigen, the primary cells to encounter it are the macrophages that phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor. Interleukin8 is often associated with inflammation. As an example, it has been cited as a proinflammatory mediator in psoriasis.1 PubMed References & Citations1. Haraldsen, G. et al. (1998) J Exp Med. 188: 1751 |