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> Anti-PAR6 Antibody (2R536)

Anti-PAR6 Antibody (2R536)

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Référence NB-64-159917-100uL

Conditionnement : 100uL

Marque : Neo Biotech


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Anti-PAR6 Antibody (2R536)

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Catalog No. TMAB-09984 Copy Product Info
Anti-PAR6 Antibody (2R536) is a Rabbit antibody targeting PAR6. Anti-PAR6 Antibody (2R536) can be used in WB,IP,FCM.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-PAR6 Antibody (2R536) is a Rabbit antibody targeting PAR6. Anti-PAR6 Antibody (2R536) can be used in WB,IP,FCM.
Ig Type
IgG
Clone
2R536
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Jurkat cell lysate. Using PAR6 (TMAB-09984) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PAR6 antibody (TMAB-09984, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
WB
IP
FCM
Recommended Dose
WB: 1:1000-2000; IP: 1:20-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Cell membrane. Cell projection, ruffle. Cell junction, tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
Tissue SpecificityExpressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundAdapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Antigen Details
Immunogen
A synthesized peptide: human PAR6 alpha
Antigen Species
Human
Gene Name
PARD6A
Gene ID
50855
Protein Name
Partitioning defective 6 homolog alpha
Uniprot ID
Q9NPB6
Function
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Chemical Properties
Molecular WeightTheoretical: 37 kDa. Actual: 37 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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