Arachis hypogaea Lectin (PNA) - Cy3

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and consists of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. PNA may be used for clinical determination of T-polyagglutinability of erythrocytes and probing cell membranes of normal and malignant cells. This Lectin can be of use in characterization of tumor-specific antigens on the surface of malignant cells. It can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

Affinity purified PNA is conjugated to cyanine 3 (Cy3) fluorescent dye. Cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media and are brighter in a non-polar environment than in an aqueous medium. Advantages of plastic over aqueous mounting media are brightness, contrast, and longevity of the fluorescence. Advantage of Cy3 conjugates are bright and photostable in the aqueous environment as well as in non-polar media. This product comes in a stabilized liquid form.

Recommended Usage: Recommended dilution is 0.5 – 10 µg/ml in 20mM Tris-HCl pH 7.5 containing 1mM CaCl2 and 0.5 mM MnCl2, 50 mM NaCl. Less than 1 µg / ml will agglutinate neuraminidase treated human erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Cell typing, Glycobiology.

References

1.Jie Sun, Qing-li Yang*, jie Bi, Chu-shu Zhang, Li-na Yu and Feng Zhu. Purification and Identification of a Natural Lectin from the Seed of Peanut Arachis hypogaea. The Open Materials Science Journal, 2011, 5, 78-82
2.Erber, W. N., Asbahr, H., Meyer, B., Herrmann, R. P., & Davies, J. M. (1992). Peanut agglutinin (lectin from Arachis hypogaea) binding to hemopoietic cells: an immunophenotypic study using a biotin streptavidin technique. Pathology, 24(3), 173–176. https://doi.org/10.3109/00313029209063168.
3.Jones, C. J., Benbow, E. W., Knox, W. F., & Stoddart, R. W. (1988). Method for showing human spermatogenesis using Arachis hypogaea (AHA) lectin. Journal of clinical pathology, 41(9), 1017–1018. https://doi.org/10.1136/jcp.41.9.1017
4.Rambihar, C., & Kerman, K. (2010). Magnetic bead-based fluorometric detection of lectin-glycoprotein interactions. Talanta, 81(4-5), 1676–1680. https://doi.org/10.1016/j.talanta.2010.03.021