General Information
| Short Description | Atypical Chemokine Receptor D6 Positive Control for STJ500034 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Specificity | This is positive control is recommended for use in combination with Atypical Chemokine Receptor D6 antibody STJ500034. |
Additional Info
| Tissue Specificity | Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors. Expressed at high levels in Kaposi sarcoma-related pathologies. Expressed on apoptotic neutrophils (at protein level). Expressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node. |
| Post Translational Modifications | Phosphorylated on serine residues in the C-terminal cytoplasmic tail. |
| Function | Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs. |
| Peptide Name | Atypical Chemokine Receptor 2C-C Chemokine Receptor D6Chemokine Receptor Ccr-10Chemokine Receptor Ccr-9Chemokine-Binding Protein 2Chemokine-Binding Protein D6 |
| Database Links | |
| Cellular Localisation | Early EndosomeRecycling EndosomeCell MembraneMulti-Pass Membrane ProteinPredominantly Localizes To Endocytic VesiclesAnd Upon Stimulation By The Ligand Is Internalized Via Clathrin-Coated PitsOnce InternalizedThe Ligand Dissociates From The ReceptorAnd Is Targeted To Degradation While The Receptor Is Recycled Back To The Cell Membrane |
| Alternative Peptide Names | Atypical Chemokine Receptor 2 proteinC-C Chemokine Receptor D6 proteinChemokine Receptor Ccr-10 proteinChemokine Receptor Ccr-9 proteinChemokine-Binding Protein 2 proteinChemokine-Binding Protein D6 proteinACKR2 proteinCCBP2 proteinCCR10 proteinCMKBR9 proteinD6 protein |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Atypical Chemokine Receptor D6 Positive Control for STJ500034 peptide (STJ503624)
This is a positive control reagent intended for use in combination with the Atypical Chemokine Receptor D6 antibody STJ500034. It is supplied ready-to-use and is specifically recommended to confirm antibody performance in western blotting experiments.
This product is validated for use in western blotting (WB) only. It is not recommended for other applications based on the available product data.
It is supplied as 100 uL ready-to-use in SDS-PAGE sample buffer (Laemmli buffer) containing Tris pH 6.8, 1% SDS, glycerol, and bromophenol blue as a tracking dye. The sample is reduced with 2% beta-mercaptoethanol, so it is ready to load directly onto a gel without further preparation.
A defined concentration value is not stated; instead, the protein concentration is empirically adjusted to produce a clear band with an appropriate signal-to-noise ratio when used with the corresponding antibody STJ500034.
This product should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain product integrity.
This product corresponds to Atypical Chemokine Receptor D6, which is listed under UniProt accession O00590. It is intended solely as a research use only reagent to support western blot validation of antibody STJ500034.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.