Bauhinia purpurea Lectin (BPL/BPA) - Cy3

Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 120 kDa. Binding appears to be highest for glycoconjugates containing galactosyl (β-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct.

Cy3 can be used to visualize the binding pattern of Bauhinia purpurea Lectin (BPL/BPA) in cellular imaging and flow cytometry. This dye is more photostable than many other fluorophores and can be detected using TRITC filter sets. Cy3 is often combined with green-fluorescent dyes for dual-labeling applications.

Recommended Usage: Recommended dilutions of 0.5 – 10 µg / ml in 1XPBS. Less than 1 µg / ml will agglutinate human erythrocyte.

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Glycan array, Biomarker, Glycobiology.

References

1. N. Martin Young, David C. Watson and Ross E. Williams. Lectins and legume chemotaxonomy. Characterization of the N-acetyl-D-galactosamine specific lectin of Bauhinia purpurea. 1985 March, Vol 182, Issue 2, 25, 403-406.
2. Yamamoto K, Konami Y, Osawa T. Determination of the carbohydrate-binding site of Bauhinia purpurea lectin by affinity chromatography. J Chromatogr. 1992 Apr 24;597(1-2):221-30.
3. Nascimento KS, Morada RCV, Oliveira MV, Martins FWV, Sacramento-Neto JC, Cavada BS. Purification and partial characterization of a new melibiose-specific lectin from Bauhinia catingae Harms. 2024 Dec, Int J Biol Macromol.;282 (Pt 1):136564.
4. Wu AM, Wu JH, Liu JH, Singh T. Recognition profile of Bauhinia purpurea agglutinin (BPA). 2004 Feb. Life Sci. 20;74(14):1763-79.