BiorbytNext ATAC-seq Library Prep Kit for Illumina

This is an ATAC-seq library construction kit developed for Illumina's high-throughput sequencing platform for 103~105 Sample building for a starting volume of one cell.ATAC-seq (Assay for Transposase Accessible Chromatin with high-throughput sequencing) is a high-throughput sequencing technology mediated by transposase for rapid detection of open regions of chromatin on a genome-wide scale; it can reveal the association between chromatin accessibility and gene expression regulation, providing key clues for epigenetic studies; it is applicable to epigenetic studies such as chromosome openness mapping, embryonic development, prediction of potential markers of diseases, tumorigenesis, etc. This product uses Tn5 transposon containing part of illumina sequencing junction, combined with chromatin open region, to cut naked DNA and add sequencing junction at the same time, and then obtain sequencing library after PCR amplification, which has the advantages of wide range of cellular starting range, short experimental period, high signal-to-noise ratio, and wide range of applications. The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change any reaction components or replace the components in this product with other equivalent products in order to avoid obtaining bad experimental results. If replacement is needed, please verify it first.