Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Biotin [58-85-5]

Imprimer

Référence HY-B0511-1g

Conditionnement : 1g

Marque : MedChemExpress

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Description

Biotin, vitamin B7 and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids^[1]^[2]^[3].

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
ES-2	IC₅₀	>100 μM Compound: Biotin	Antiproliferative activity against human ES2 cells Antiproliferative activity against human ES2 cells	[PMID: 38716896]
HEK-293T	IC₅₀	>100 μM Compound: Biotin	Antiproliferative activity against HEK293T Antiproliferative activity against HEK293T	[PMID: 38716896]
OVCAR-3	IC₅₀	>100 μM Compound: Biotin	Antiproliferative activity against human OVCAR-3 cells Antiproliferative activity against human OVCAR-3 cells	[PMID: 38716896]

In Vitro

Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with K_ms of 9.24 μM and 53.1 μM, respecetively^[4].
Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with V_max of 27.34 pmol/mg protein/min^[4].
Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 µM)-induced cell death and reduces cell adhesion^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[5]

Cell Line:	Murine oligodendrocyte 158N cells
Concentration:	1, 10, 100, 1000 nM
Incubation Time:	24 hours
Result:	Showed cytoprotective effects and prevents 7β-hydroxycholesterol-induced disruption of redox status. Improved attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration.

In Vivo

Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice^[6].
Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Streptozotocin-induced Diabetes type 1 in male Swiss albino mice (25±2 g)^[6]
Dosage:	15 mg/kg/d
Administration:	Oral gavage; 12 days
Result:	Improved histopathological results, including distorted glomeruli, inflammatory cells, and giant macrophages, and diminished acroline reaction of oxidative damage.

Animal Model:	Grass carp (117±0.5 g)^[7]
Dosage:	0.012, 0.110, 0.214, 0.311, 0.427, and 0.518 mg/kg
Administration:	Oral gavage; 70 days
Result:	Reduced the activities of lysozyme (LZ) and acid phosphatase (ACP), decreased the contents of complement 3 (C3), C4 and immunoglobulin M (IgM). Reduced the mRNA levels of anti-microbial substances. Increased the mRNA levels of pro-inflammatory cytokines, tumour necrosis factor partially and reduced anti-inflammatory IL-4/13A, IL-10, IL-11 and TGF-β1 mRNA levels partially in association with target of rapamycin (TOR) signaling.

Masse moléculaire

244.31

Formule

C₁₀H₁₆N₂O₃S

CAS No.

58-85-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CCCC[C@@H]1SC[C@]([C@]1(20)N2)(20)NC2=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (409.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0932 mL	20.4658 mL	40.9316 mL
5 mM	0.8186 mL	4.0932 mL	8.1863 mL
10 mM	0.4093 mL	2.0466 mL	4.0932 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 1.96 mg/mL (8.02 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

Pureté et documentation

Purity: 99.80%

Fiche technique (281 KB) SDS (396 KB)

COA (251 KB) HNMR (206 KB) LCMS (386 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Zempleni, et al. Biotin biochemistry and human requirements. J Nutr Biochem, 1999. 10(3): p. 128-38. [Content Brief]

[2]. Mock, D.M.. Biotin status: which are valid indicators and how do we know? J Nutr, 1999. 129(2S Suppl): p. 498S-503S. [Content Brief]

[3]. Zempleni J, et al. Biotin. Biofactors. 2009 Jan-Feb;35(1):36-46. [Content Brief]

[4]. Vadlapudi AD, et al. Biotin uptake by T47D breast cancer cells: functional and molecular evidence of sodium-dependent multivitamin transporter (SMVT). Int J Pharm. 2013 Jan 30;441(1-2):535-43. [Content Brief]

[5]. Sghaier R, et al. Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes. Free Radic Res. 2019 May;53(5):535-561. [Content Brief]

[6]. Aldahmash BA, et al. Biotin amelioration of nephrotoxicity in streptozotocin-induced diabetic mice. Saudi J Biol Sci. 2015 Sep;22(5):564-9. [Content Brief]

[7]. He P, et al. Dietary biotin deficiency decreased growth performance and impaired the immune function of the head kidney, spleen and skin in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2020 Feb;97:216-234. [Content Brief]

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Description

Cond.

Prix HT

Biotin [58-85-5]

Référence HY-B0511-1g

Marque : MedChemExpress

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