Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:10000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:1000.

Calnexin antibody detects Calnexin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Calnexin protein stained by Calnexin antibody (GTX112886) diluted at 1:200.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX112886 diluted at 1:10000

Calnexin antibody detects Calnexin protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Calnexin antibody (GTX112886) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calnexin(GTX112886) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX112886 diluted at 1:1000

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.