Anti-CD21 (CR2, Complement Receptor Type 2) (FITC) Monoclonal Antibody

Référence 397002-100ug

Conditionnement : 100ug

Marque : US Biological

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397002 CD21 (CR2, Complement Receptor Type 2) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
bovine
Swiss Prot
Q8HY44
Isotype
IgG2b
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

This is a 145kD single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells. CD21 may be expressed on B-cells as either a long or a short form. Mouse anti Bovine CD21, demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells. In addition this has been demonstrated to recognise CD21 in a range of ruminant and other species is also available.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution: |Flow Cytometry: Neat - 1:10|Optimal dilutions to be determined by the researcher.||Hybridoma:|NSI myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2b|Clone No: CC51|Host: mouse|Source: bovine|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Bovine (Friesian cattle) mesenteric lymph node cells|Specificity: Recognizes bovine CD21. Species Crossreactivity: porcine, african buffalo.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Bovine (Friesian cattle) mesenteric lymph node cells
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes bovine CD21. Species Crossreactivity: porcine, african buffalo.
References
1. Sinkora, M. et al. (2014) The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2. Vet Res. 45: 91.|2. Sinkora, M et al. (2013) Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs. Dev Comp Immunol. 39: 409-18.|3. Tenaya I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus. Vet Immunol Immunopathol. 149: 167-76.|4. Denham S. et al. (1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells. Vet Immunol Immunopathol. 43: 259-67.|5. Boersma W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers. Vet Immunol Immunopathol. 80: 63-78.|6. Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes. Immunology. 69 (4): 525-30.