Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Human MG-63 lysates probed with Rabbit Anti-P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

MCF-7 cells were stained with P21 Polyclonal Antibody, Unconjugated(bs-10129R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

Paraformaldehyde-fixed, paraffin-embedded Human Hepatoma tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody, DAB and counterstaining.

Tissue\/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CDKN1A\/P21) Polyclonal Antibody, Unconjugated (bs-10129R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.