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> Chicken Interleukin 10,IL-10 ELISA Kit

Chicken Interleukin 10,IL-10 ELISA Kit

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Référence CSB-E12835C-24T

Conditionnement : 24T

Marque : Cusabio


CSB-E12835C
Size 96T,5×96T,10×96T

Product Details

Target Name
interleukin 10
Associated names
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit
Abbreviation
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1 pg/mL-200 pg/mL
Sensitivity
0.5 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-101  
1:2 Average % 97  
Range % 94-105  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 92  
Range % 87-96  
Recovery
The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 97 93-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average 　  
200 0.155 0.152 0.154 　  
50 0.334 0.348 0.341 　  
16 0.515 0.502 0.509 　  
4 0.969 0.975 0.972 　  
1 1.436 1.463 1.450 　  
0 2.658 2.667 2.663 　  
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Chicken IL10 ELISA Kit was designed for the quantitative measurement of Chicken IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1 pg/mL-200 pg/mL and the sensitivity is 0.5 pg/mL.

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Target Background

Function
(From Uniprot)
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including...
Subcellular Location
Secreted.
Protein Families
IL-10 family
Tissue Specificity
Expressed predominantly in bursa of Fabricius and cecal tonsils with low levels in thymus, liver and lung.
Database Links

KEGG:

STRING:

UniGene: