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> Chicken Interleukin 12,IL-12/70 ELISA Kit

Chicken Interleukin 12,IL-12/70 ELISA Kit

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Référence CSB-E12836C-24T

Conditionnement : 24T

Marque : Cusabio


CSB-E12836C
Size 96T,5×96T,10×96T

Product Details

Target Name
Interleukin 12,IL-12/70
Abbreviation
IL12/70
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-12/70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 95  
Range % 88-98  
1:2 Average % 94  
Range % 90-98  
1:4 Average % 92  
Range % 87-98  
1:8 Average % 96  
Range % 92-101  
Recovery
The recovery of chicken IL-12/70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-99  
EDTA plasma (n=4) 92 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.192 0.188 0.190    
50 0.273 0.281 0.277    
12.5 0.585 0.565 0.575    
3.12 0.876 0.824 0.850    
0.78 1.424 1.457 1.441    
0 2.265 2.233 2.249    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Chicken IL12/70 ELISA Kit was designed for the quantitative measurement of Chicken IL12/70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.78 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

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