Chicken Interleukin 6,IL-6 ELISA Kit
Référence CSB-E08549Ch-24T
Conditionnement : 24T
Marque : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
interleukin 6 (interferon, beta 2)
Associated names
IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-100 | |||||
Recovery
| The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 91-105 | ||||
| EDTA plasma (n=4) | 96 | 88-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Chicken IL6 ELISA Kit was designed for the quantitative measurement of Chicken IL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
Citations
- L Yao, H Wang, L Li, Q Xiao, Z Zeng, Y Shang, H Feng,Animals,2026
- H Jiang, J Zhang, K Zhang, X Lu, Z Guo, G Xu, H Chen,Poultry Science,2026
- W Han, L Chen, L Wang, Q Zhao, S Zhu, Y Yu, J Bai,Poultry Science,2026
- M Al-mansouri,Animal and Veterinary Journal,2026
- L Huang, R Qin, Q Yu, Q Yan, D Qi,Animals,2026
- Substitution of Soybean Meal with Black Soldier Fly (Hermetia illucens) Larvae Meal in Broiler Diets: Comprehensive Effects on Growth, Gut Health and Physiological Resilience AO Abbas, NK Al-Suwailem, AA Alaqil, A Assiri,PAKISTAN VETERINARY JOURNAL,2026
- G Zhang, F Tang, Y Wang, H Liu,Animals,2026
- W Yang, X Liu, G Chen, C Duan, H Zhang, Y Chen,Poultry Science,2025
- Z Li, H Xu, Z Tan, H Wang, Z Li, Y Luo, Y Li, Y Chen,Poultry Science,2025
- VO Chimezie, KM Okukpe, OI Alli,Zanco Journal of Pure and Applied Sciences,2025
Customer Reviews and Q&A
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Target Background
Function
(From Uniprot)
(From Uniprot)
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
Gene References into Functions
- Avian leukosis virus subgroup J induces VEGF expression via NF-kappaB/PI3K-dependent IL-6 production to promoter tumorigenesis.
- The effects of daily corticosterone injections on acute phase proteins (APP), IL-6, and HSP70 are reported; APP and HSP70 may be of value as indicators of stress in poultry.
- Chicken IL-6 is a heat-shock gene
- Nampt/visfatin/PBEF affects expression of myogenic regulatory factors and is regulated by interleukin-6 in chicken skeletal muscle cells
- IL-6 is involved in the progression of chicken arthritis induced by Staph. aureus, and it contributes to disease incidence and mortality
Subcellular Location
Secreted.
Protein Families
IL-6 superfamily
Database Links
KEGG:
UniGene: