Conditionnement : 10g
Synonyms: Levomycetin, Chloromycetin, Chlornitromycin
| Description | Chloramphenicol (Chloromycetin) is a broad-spectrum antibiotic that inhibits the biosynthesis of bacterial proteins. Chloramphenicol acts primarily on the 50S subunit of the bacterial 70S ribosome and is active against peptidyltransferases, inhibiting the formation of peptide bonds. |
| Targets & IC50 | protozoan motility:2.95 ± 0.25 mM |
| In vitro | METHODS: Human lung cancer cells A549 and H1299 were treated with Chloramphenicol (1-100 µg/mL) for 3-24 h. Cell viability was detected by MTT assay. RESULTS: In the 3 h treatment group, the viability of A549 and H1299 cells at 100 µg/mL was 97.0±3.9% and 98.1±5.0%, respectively, and no cytotoxicity was observed. A significant reduction in cell viability was observed in the 24 h treatment group. [1] METHODS: Mouse T cell lines A1.1 and IE5 and human T cell lines Jurkat and Jcam were stimulated with Chloramphenicol (100 nmol/L), and the DNA content was measured by Flow cytometry. RESULTS: Chloramphenicol clearly blocked activation-induced cell death (AICD) in all four cell lines, even though these cells were activated by different stimuli. [2] |
| In vivo | METHODS: To investigate the potential to induce aplastic anemia, Chloramphenicol (2000 mg/kg) and Thiamphenicol (850 mg/kg) were administered by gavage to BALB/c mice once daily for 17 days. RESULTS: Administration of Chloramphenicol and Thiamphenicol at hematotoxic dose levels for 17 days in BALB/c mice was associated with the development of aplastic anemia in humans, inducing reversible anemia but not chronic bone marrow aplasia. [3] |
| Synonyms | Levomycetin, Chloromycetin, Chlornitromycin |
| Molecular Weight | 323.13 |
| Formula | C11H12Cl2N2O5 |
| Cas No. | 56-75-7 |
| Smiles | [C@@H]([C@H](NC(C(Cl)Cl)=O)CO)(O)C1=CC=C(N(=O)=O)C=C1 |
| Relative Density. | 1.547 g/cm3 |
| Storage | Keep away from direct sunlight,Store under nitrogen Powder: -20°C for 3 years Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: 1 mg/mL (3.09 mM), Sonication is recommended.  Ethanol: 60 mg/mL (185.68 mM), Sonication is recommended. DMSO: 250 mg/mL (773.68 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (15.47 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
H2O/Ethanol/DMSO
Ethanol/DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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