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> Chlorothalonil [1897-45-6]

Chlorothalonil [1897-45-6]

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Référence HY-N6625-500mg

Conditionnement : 500mg

Marque : MedChemExpress


Description

Chlorothalonil is a broad-spectrum foliar fungicide with oral activity. Chlorothalonil can be used to combat fungal diseases in vegetable and crop leaves. Chlorothalonil can alter the microbial community in the soil. Chlorothalonil inhibits spermatogenesis. Chlorothalonil can cause intestinal epithelial barrier dysfunction and fetal toxicity[1][2][3][4][5].

In Vitro

Chlorothalonil (10mg/kg soil) inhibits the activity of phosphatase and dehydrogenase activity in soil, reduces the number of microorganisms in soil[3].
Chlorothalonil (0.6-4.8 µg/mL; 4 days) induces intestinal epithelial barrier (IEB) dysfunction in Caco-2 cells by activating the mitogen-activated protein kinase (MAPK) pathway[4].
Chlorothalonil (0.1-10 μM; 4 h and 24 h) reduces porcine sperm motility and increases apoptosis in a concentration- and time-dependent manner[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chlorothalonil Related Antibodies

RT-PCR[4]

Cell Line: Caco-2 (Human colorectal adenocarcinoma cell line)
Concentration: 0.6 µg/mL, 1.2 µg/mL , 2.4 µg/mL, 4.8 µg/mL
Incubation Time: 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 8 days
Result: Down-regulated the mRNA levels of TJ genes (ZO-1, OCLN, and CLDN1), polar marker genes (SI), and anti-apoptotic genes (BCL-2), and up-regulated the mRNA levels of apoptosis-related genes (BAD, BAX, CASP3, and CASP8).

Western Blot Analysis[4]

Cell Line: Caco-2 (Human colorectal adenocarcinoma cell line)
Concentration: 0.6 µg/mL, 1.2 µg/mL , 2.4 µg/mL
Incubation Time: 4 days
Result: Decreased levels of the TJ protein network (ZO-1 and CLDN1).
Increased the expression levels of P-ERK1/2, PJNK and P-p38 and reduced levels of ERK1/2, JNK and p38.( All are mitogen-activated protein kinase (MAPK) signaling pathway-related proteins)
In Vivo

Chlorothalonil (400-600 mg/kg; p.o.; Once daily for 18 days) produces toxicity and fetal toxicity in pregnant ICR mice[1].
Chlorothalonil (0.1-10 mg/kg; p.o.; Once daily for 5 weeks) reduces sperm motility and inhibits spermatogenesis in ICR mice[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR mouse model of pregnancy[1]
Dosage: 100 mg/kg, 400 mg/ kg, 600 mg/kg
Administration: Oral gavage (p.o.); Once daily for 18 days
Result: Reduced weight gain in pregnant mice at doses of 400 mg/kg and 600 mg/kg throughout pregnancy by 36% and 48%, respectively.
Significantly increased embryo mortality at doses of 400 mg/kg and 600 mg/kg.
Significantly reduced number of live births (28%).
Reduced mean fetal weight (22% to 39%) at doses of 400 and 600 mg/kg.
Animal Model: ICR male mice model[5]
Dosage: 0.1mg/kg, 1 mg/ kg, 10 mg/kg
Administration: Oral gavage (p.o.); Once daily for 5 weeks
Result: Decreased sperm motility in ICR mice.
At doses of 1 mg/kg and 10 mg/kg, decreased the protein factors A-myb, GDNF and DDX4, and finally interrupted the spermatogenesis process.
Decreased the sperm marker Acrosin and PNA in a dose-dependent manner at doses of 1 mg/kg and 10 mg/kg.
At doses of 1 mg/kg and 10 mg/kg, Significantly reduced the number of estrogen receptor Alpha (ERα) positive stromal cells in mouse testis.
Decreased AKT levels in the testis, but did not change p-AKT levels.
Disrupted the methylation of histones and DNA.
Masse moléculaire

265.91

Formule

C8Cl4N2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N#CC1=C(Cl)C(Cl)=C(Cl)C(C#N)=C1Cl
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvant et solubilité
In Vitro: 

DMSO : 33.33 mg/mL (125.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7607 mL 18.8034 mL 37.6067 mL
5 mM 0.7521 mL 3.7607 mL 7.5213 mL
View the Complete Stock Solution Preparation Table

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.40 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (9.40 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Pureté et documentation
Références