Cholic acid [81-25-4]

Biological Activity

Cholic acid is a major primary bile acid produced in the liver and usually conjugated with glycine or taurine. It facilitates fat absorption and cholesterol excretion.

Protocol (for reference only)

Cell Experiment
Cell lines	HepG2 cells
Preparation method	HepG2 cells are pretreated with the inhibitors NaN3 (1 mg/mL), Genistein (50 μg/mL), MβCD (10 mM), Nystatin (50 μg/mL), Chlorpromazine (10 μg/mL), and Cholic acid (1 mg/mL) for 30 min. After removing the inhibitors, the cells are incubated with CA-LPs for 2 h, and the cellular uptake of LPs is determined in the "In vitro cellular uptake assays" section.
Concentrations	1 mg/mL
Incubation time	30 min

Animal Experiment
Animal models	Tg-CYP2D6 mice
Formulation	1% (w/w) Cholic acid-supplemented
Dosages	14 days
Administration	fed

Chemical Information

Molecular Weight	408.57
Formula	C24H40O5
CAS Number	81-25-4
Solubility (25°C)	DMSO: ≥ 40 mg/mL
Storage	4°C, dry, sealed

References

[1] Li Y, et al. Int J Nanomedicine. Mechanism of hepatic targeting via oral administration of DSPE-PEG-cholic acid-modified nanoliposomes.