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> COVID-19 Human IgG ELISA Kit

COVID-19 Human IgG ELISA Kit

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Référence IEQ-CoVS1RBD-IgG-1

Conditionnement : 1PlateKit

Marque : RayBiotech


IEQ-CoVS1RBD-IgG1 was used to quantify anti-S1 receptor-binding domain IgG antibodies in plasma samples from cancer patients with COVID-19. Figure adapted from Remsik et al., Cancer Cell, 2021.IEQ-CoVS1RBD-IgG was used to quantify spike S1–specific IgG antibodies in matched maternal and fetal cord plasma from SARS-CoV-2–infected or vaccinated pregnancies. Figure adapted from Monroe et al., Journal of Infectious Diseases, 2024.IEQ-CoVS1RBD-IgG was used to quantify SARS-CoV-2 S1 RBD–specific IgG antibodies in serum from vaccinated animals, showing increased RBD-binding IgG titers after immunization. Figure adapted from Reguzova et al., NPJ Vaccines, 2024.IEQ-CoVS1RBD-IgG was used to quantify SARS-CoV-2 spike RBD levels in COVID-19 patient serum samples by ELISA. Figure adapted from Das et al., Microbiology and Immunology, 2024.The kit was used to quantify spike S1–specific IgG antibodies in matched maternal and fetal cord plasma collected at delivery from SARS-CoV-2–infected or vaccinated pregnancies. Figure adapted from Monroe et al., Journal of Infectious Diseases, 2024.COVID-19 Human IgG ELISA KitCOVID-19 Human IgG ELISA KitCOVID-19 Human IgG ELISA KitCOVID-19 Human IgG ELISA Kit

IEQ-CoVS1RBD-IgG1 was used to quantify anti-S1 receptor-binding domain IgG antibodies in plasma samples from cancer patients with COVID-19. Figure adapted from Remsik et al., Cancer Cell, 2021.

COVID-19 Human IgG ELISA Kit

RayBio® COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Compatible Sample TypesPlasma, Serum, Dried Blood, Saliva
Gene Symbols
IGG
Quantitative/Semi-QuantitativeSemi-Quantitative
Solid Support
96-well Microplate
Design PrincipleIndirect
Method Of DetectionColorimetric
Research AreaInfectious Disease, COVID-19
Shipping TypeDry ice (additional shipping fee)
Storage-80°C
NoteThis Product is for Research Use Only, not for use in in vitro diagnostics, following guidance from the FDA for tests submitted to the FDA for Emergency Use Authorization and Notification on March 16th, 2020, and amended on May 4th, 2020. While this test has been submitted for FDA EUA Authorization, it currently has not been reviewed by the FDA and results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status. Please consult the manual for specific details and limitations of this test.
AntigenN and S (S1RBD), N-Protein, S-Protein (S1RBD)

Typical Data

IEQ-CoVS1RBD-IgG1 was used to quantify anti-S1 receptor-binding domain IgG antibodies in plasma samples from cancer patients with COVID-19. Figure adapted from Remsik et al., Cancer Cell, 2021.

IEQ-CoVS1RBD-IgG-1 was used to quantify spike S1–specific IgG antibodies in matched maternal and fetal cord plasma from SARS-CoV-2–infected or vaccinated pregnancies. Figure adapted from Monroe et al., Journal of Infectious Diseases, 2024.

IEQ-CoVS1RBD-IgG-5 was used to quantify SARS-CoV-2 S1 RBD–specific IgG antibodies in serum from vaccinated animals, showing increased RBD-binding IgG titers after immunization. Figure adapted from Reguzova et al., NPJ Vaccines, 2024.

IEQ-CoVS1RBD-IgG-1 was used to quantify SARS-CoV-2 spike RBD levels in COVID-19 patient serum samples by ELISA. Figure adapted from Das et al., Microbiology and Immunology, 2024.

IEQ-CoVS1RBD-IgG-1 was used to quantify spike S1–specific IgG antibodies in matched maternal and fetal cord plasma collected at delivery from SARS-CoV-2–infected or vaccinated pregnancies. Figure adapted from Monroe et al., Journal of Infectious Diseases, 2024.

Kit Components

  • SARS-CoV-2 N-protein coated 96-well microplate
  • Wash Buffer
  • Positive Control
  • Biotinylated Anti-Human IgG
  • HRP-Streptavidin concentrate
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diluent
  • 5X Sample Diluent
  • 1X PBS (DBS version)
  • Tween-20 (DBS version)
  • Protease Inhibitor Cocktail (DBS version)

Additional Materials Required

  • Microplate reader capable of measuring absorbance at 450 nm
  • Precision pipettes to deliver 2 µL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Distilled or deionized water

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 µL positive control or sample to each well. Incubate 1 hour at room temperature.
  3. Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
  4. Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
  5. Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
  6. Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

citations

  • Inflammatory Leptomeningeal Cytokines Mediate COVID-19 Neurologic Symptoms in Cancer Patients

    Species:
    Human
    Sample Type:
    Plasma, CSF (Inflammation in the brain during covid)
    Year:
    2021
    Journal Name:
    Cancer Cell
    PubMed ID:
    33508216

  • Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics

    Sample Type:
    Magnetic nanoparticles (MNPs)
    Year:
    2022
    Journal Name:
    Nature Communications

  • Vertical Transmission of SARS-CoV-2-Specific Antibodies and Cytokine Profiles in Pregnancy

    Year:
    2024
    Journal Name:
    Journal of Infectious Diseases
    PubMed ID:
    37786979

  • A multiantigenic Orf virus-based vaccine efficiently protects hamsters and nonhuman primates against SARS-CoV-2

    Year:
    2024
    Journal Name:
    NPJ vaccines
    PubMed ID:
    39414789

  • Severe acute respiratory syndrome coronavirus 2-reactive salivary antibody detection in South Carolina emergency healthcare workers, September 2019-March 2020

    Year:
    2024
    Journal Name:
    Epidemiology and Infection
    PubMed ID:
    39320488

  • N-glycosylation of the SARS-CoV-2 spike protein at Asn331 and Asn343 is involved in spike-ACE2 binding, virus entry, and regulation of IL-6

    Year:
    2024
    Journal Name:
    Microbiology and Immunology
    PubMed ID:
    38444370

  • CD8+ T cell memory induced by successive SARS-CoV-2 mRNA vaccinations is characterized by shifts in clonal dominance

    Year:
    2024
    Journal Name:
    Cell Rep
    PubMed ID:
    38458195

  • Vertical Transmission of SARS-CoV-2–Specific Antibodies and Cytokine Profiles in Pregnancy

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Journal of Infectious Diseases

  • Refocus on Immunogenic Characteristics of Convalescent COVID-19 Challenged by Prototype SARS-CoV-2

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Vaccines

  • Exosome-Based Multivalent Vaccine: Achieving Potent Immunization, Broadened Reactivity, and Strong T-Cell Responses with Nanograms of Proteins

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Microbiology spectrum

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