Background: Cancer metastasis comprises several steps. First tumor cells are shed into the blood stream (intravasation), circulating in the blood, and finally transmigrating out of the vessels (extravasation) into a new location in the body. The initial arrest and attachment of tumor cells to vascular endothelium precedes their extravasation from the blood stream and is a crucial step in the tumor metastatic cascade. Tumor cell extravasation is equivalent, in many respects, to the entry of leukocytes into inflammatory tissue. Leukocyte extravasation consists of multiple, consecutive processes including the capture of circulating leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration. Increasing evidence suggests that tumor cell adhesion to the endothelial lining and transendothelial migration is influenced by endothelial activation or tissue-specific differences in endothelium and depends on the expression of specific cell surface molecules. E-Selectin and Vascular Cell Adhesion Molecule-1 (VCAM-1) appear to play a pivotal role in the tumor-EC interaction. CytoSelect Tumor Transendothelial Migration Assay provides a robust system for the quantitative determination of tumor-endothelium interactions and transmigrations. The kit contains sufficient reagents for the evaluation of 24 assays in a 24-well plate.

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Small volumes of CytoSelect Tumor Transendothelial Migration Assay assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169012 is an assay kit targeting CytoSelect Tumor Transendothelial Migration Assay.