DDX4 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 4, DEAD Box Protein 4, Probable ATP-dependent RNA Helicase DDX4, Vasa Homolog, VASA)

DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to the germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer suggesting its role in the microRNA-mediated
RNA silencing (1).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.