DNA ligase III antibody [1F3]
![Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFg3MDE0My9HVFg3MDE0M18yNjg4MV9XQl93XzIzMDYxMjIxXzE5My53ZWJw.webp)
The indicated band with observed MW (103 kD) may represent the larger isoform of human DNA Ligase III as reported by Chen J et al and Nash RA et al.
DNA ligase III detects DNA ligase III antibody [1F3] protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
DNA ligase III (GTX70143) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFg3MDE0My9HVFg3MDE0M180MTgxM19XQl93XzIzMDYxMjIxXzM4MC53ZWJw.webp)
DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX70143) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFg3MDE0My9HVFg3MDE0M18yMDI0MDQxMV9jaXRlYWJfV0JfMjcyOV8yNDA0MTUwMl8yMDMud2VicA==.webp)
The data was published in the journal PLoS One in 2013. PMID: 23555685