DNA Polymerase T4
Référence MBS653894-100U
Conditionnement : 100Units
Marque : MyBiosource
DNA Polymerase T4 (DNA) Enzyme
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653894Catalyzes 5'=>3' synthesis of DNA. Enzyme has 3'=>5' exonuclease activity but lacks 5'=>3' exonuclease activity. T4 DNA polymerase possessing 3'=>5' exonuclease activity exhibits greater activity on single-stranded DNA than on double-stranded DNA. More details
Product Overview
Online Datasheet in PDFProduct Name
DNA Polymerase T4, Enzyme
Full Product Name
DNA Polymerase T4
Form/Format
Supplied as a liquid in 20mM potassium phosphate, pH 7.5, 200mM potassium chloride, 2mM DTT, 50% glycerol.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
~104kD
Quality Control
No conversion of covalently closed circular DNA to nicked DNA was detected after incubation of 10 units of T4 DNA Polymerase with 1ug of pUC19 DNA in 50ul reaction buffer for 4 hours at 37 degree C.
Unit Definition
One unit of T4 DNA Polymerase catalyzes the incorporation of 10nmoles of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37 degree C.
Product Categories/Family for DNA Polymerase T4 enzyme
Cloning; Cloning-Enzymes
Application Notes for DNA Polymerase T4 enzyme
Blunting of DNA ends: fill-in 5'-overhangs or/and removal of 3'-overhangs, see protocol, , Blunting of PCR products with 3'-dA overhangs., , Synthesis of labeled DNA probes by the replacement reaction., , Oligonucleotide-directed site-specific mutagenesis., , Ligation-independent cloning of PCR products.
Other Notes
Small volumes of DNA Polymerase T4 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DNA Polymerase T4 purchase
MBS653894 is an enzyme targeting DNA Polymerase T4.