K1136-10 | Dual Luciferase Assay System Clinisciences

Description

The dual luciferase reporter gene system is one of the important means to study the regulatory expression of genes. This kit contains high-purity firefly Luciferin and Coelenterazine, which emit bioluminescence of the corresponding wavelength during the reaction, where the firefly fluorescein emits a yellow-green light with a wavelength of 550-570 nm in the presence of firefly luciferase, oxygen, ATP and magnesium ions at the same time, while Coelenterazine can react to emit a blue light with a wavelength of 480 nm in the presence of Renilla luciferase and oxygen.

In the experiment, the expression of firefly Luciferase (Firefly Luciferase) was detected by fluorescein as a substrate, and then the fluorescence signal of firefly luciferase was quenched, and the expression of Renilla luciferase was detected with luciferin as the substrate to achieve dual luciferase reporter gene detection.

This product does not need to lyse the cells with lysate in advance, and then add fluorescence detection reagents, simplifying the experimental process. Luciferase reagent is added directly to the cell culture when used, the cells are lysed, and a firefly luciferase substrate is provided at the same time, which makes this product more suitable for high-throughput detection. Our reagents are suitable for mammalian cell culture media, such as the following types of media containing 1-10% serum: RPMI 1640, DMEM, MEMα and F12, etc.

Quality Control

Protocol

Related Biological Data

Related Biological Data

Components and Storage

Component	Size
Luciferase Buffer	10 ml
Luciferase Substrate (lyophilized)	1 vial
Stop & Glo Buffer	10 ml
Stop & Glo Substrate (100×)	100 μl
Long-term storage: -20 °C, away from light. Luciferase Buffer, Luciferase Substrate (lyophilized) and Stop & Glo Buffer are stable for 1 year. Stop & Glo Substrate (100×) is stable for 6 months.

Dual Luciferase Assay System

Référence K1136-10

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Description

Quality Control

Related Biological Data

Related Biological Data

Components and Storage

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