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> E. coli J5 LPS Mouse Monoclonal Antibody [Clone ID: 2D7/1]

E. coli J5 LPS Mouse Monoclonal Antibody [Clone ID: 2D7/1]

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Référence BM1091

Conditionnement : 100ug


E. coli J5 LPS Mouse Monoclonal Antibody [Clone ID: 2D7/1]

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SKU
BM1091
E. coli J5 LPS mouse monoclonal antibody, clone 2D7/1, Purified
Size:
100 ug
Conjugation:
Unconjugated
 

View other "J5 LPS" antibodies

Specifications
Product Data
Clone Name 2D7/1
Application ELISA, IF, WB
Recommended Dilution Immunofluorescence.
ELISA: (a 1 mg/ml solution has been used at dilutions of up to 1/1500).
Western Blotting.
Reactivity Escherichia coli
Antibody Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Escherichia coli J5 cells.
Specificity The antibody recognizes E. coli J5 LPS.
It has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.
Buffer PBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock.
The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
Synonyms Escherichia coli, EHEC, EPEC, ETEC
Reference Data
Product Manuals
FAQs
  • Antibody and Western Standard
SDS

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