The innovative EASYspin plus system is ideal or rapid purification of genomic DNA-free total RNA from bacteria. The unique genomic DNA elimination columns effectively remove genomic DNA from RNA samples. No DNase digestion step is required. The purified total RNA can be directly used for reverse transcription and RT-PCR. The whole process is phenol/chloroform-free. The unique lysis buffer immediately lyses biological samples and inactivates RNase and DNase, Centrifugal precipitation to remove polysaccharides, polyphenols and secondary metabolites, and then lyse the mixture with ethanol to adjust RNA binding and adsorption to the genomic DNA removal column, then the RNA is selectively eluted and filtered, and the residual DNA adsorbed on the genomic DNA removal column cannot be eluted Throw away together with the column to remove DNA. Ethanol is added to the lysate to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica-membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica membrane with RNase-free H2O.

Small volumes of RNA (For Bacteria)(with DNA removation columns) kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS355951 is a kit targeting RNA (For Bacteria)(with DNA removation columns). Also known as EASYspin RNA Isolation Kit (For Bacteria)(with DNA removation columns).