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Ecdysone [3604-87-3]

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Référence NB-64-64461-10mg

Conditionnement : 10mg

Marque : Neo Biotech

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Ecdysone

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Synonyms:

Catalog No. TN3910 Copy Product Info

Purity: 99.22%

Ecdysone is a major steroid hormone in insects and herbs. Ecdysone triggers mineralocorticoid receptor activation and induces cellular apoptosis. Ecdysone signaling through Ecdysone receptor isoform B1 is required cell autonomously for the muscle death.

Cas No. 3604-87-3

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.22%

Appearance:Solid

Color:White

Product Introduction

Bioactivity

Description	Ecdysone is a major steroid hormone in insects and herbs. Ecdysone triggers mineralocorticoid receptor activation and induces cellular apoptosis. Ecdysone signaling through Ecdysone receptor isoform B1 is required cell autonomously for the muscle death.
In vitro	Ecdysone (10, 100 nM) induced the expression of a-smooth muscle actin (SMA). Ecdysone elevated the expression of cleaved caspase 3 in a dose dependent manner. Ecdysone (10, 100 nM) suppressed cell motility and scratch wound closure to a comparable extent[4]. Ecdysone (100 nM) induced a branched spindle mesenchymal-like cell shape. Ecdysone (100 nM) caused renal tubular inner medullary collecting duct cells apoptosis[4].
In vivo	In male C57BL/6 mice, Ecdysone (6 μg/g/day; s.c.) significantly impaired renal function, and BUN levels were statistically significantly increased, amplifying the renal expression of α-SMA. Ecdysone also displayed MR-dependent nephrotic effects[4].

Chemical Properties

Molecular Weight	464.63
Formula	C₂₇H₄₄O₆
Cas No.	3604-87-3
Smiles	C[C@H]([C@@H](CCC(C)(C)O)O)[C@@H](CC1)[C@@](C)(CC[C@H]([C@@](C)(C[C@@H]2O)[C@H]3C[C@H]2O)C2=CC3=O)[C@]12O
Relative Density.	1.22g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (129.14 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (2.15 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1523 mL	10.7613 mL	21.5225 mL	107.6125 mL
5 mM	0.4305 mL	2.1523 mL	4.3045 mL	21.5225 mL
10 mM	0.2152 mL	1.0761 mL	2.1523 mL	10.7613 mL
20 mM	0.1076 mL	0.5381 mL	1.0761 mL	5.3806 mL
50 mM	0.0430 mL	0.2152 mL	0.4305 mL	2.1523 mL
100 mM	0.0215 mL	0.1076 mL	0.2152 mL	1.0761 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

α-EcdysonetransitionssteroidSodiumChannelSodium ChannelsleepregulationreceptorPotassiumChannelPotassium ChannelMineralocorticoid ReceptormineralocorticoidmetaboliteInhibitorinhibitHuman Endogenous MetabolitehormonehomeostaticGlucocorticoidReceptorGlucocorticoid ReceptorEndogenousMetaboliteEndogenous MetaboliteEcdysonedevelopmentalCrustecdysonecoordinatingAutophagyATPaseApoptosis20-hydroxyecdysone20E

Related Tags: Ecdysone chemical structure | Ecdysone in vivo | Ecdysone in vitro | Ecdysone formula | Ecdysone molecular weight