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> Fibrinogen (Coagulation Factor I) (FITC)

Fibrinogen (Coagulation Factor I) (FITC)

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Référence F4199-91L-1ml

Conditionnement : 1ml

Marque : US Biological


F4199-91L Fibrinogen (Coagulation Factor I) (FITC)

Clone Type
Polyclonal
Host
goat
Source
human
Isotype
IgG
Grade
Highly Purified
Applications
FLISA
Crossreactivity
Bo Ca Fe Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking.

Applications:
Suitable for use in FLISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.

Recommended Dilution:
FLISA: 1:20-1:80
Optimal dilutions to be determined by the researcher.

Excitation:
492nm

Emission:
515nm

Fluorescein/IgG protein Molar Ratio (F/P):
~1.5

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: Not determined|Form: Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.|Purity: Purified by DEAE chromatography.|Immunogen: Fibrinogen isolated from fresh plasma after removing prothrombin.|Specificity: Recognizes human fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Species Crossreactivity: Bovine, canine, feline, monkey||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Fibrinogen isolated from fresh plasma after removing prothrombin.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Purity
Purified by DEAE chromatography.
Specificity
Recognizes human fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Species Crossreactivity: Bovine, canine, feline, monkey