Fish hepatocyte growth factor(HGF)ELISA Kit
Référence CSB-E16527Fh-24T
Conditionnement : 24T
Marque : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
Abbreviation
Uniprot No.
Species
Fish
Sample Types
serum, plasma
Detection Range
230 pg/mL-8000 pg/mL
Sensitivity
145 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of fish HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 89-105 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-100 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 82-100 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 87-99 | ||||||
Recovery
| The recovery of fish HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 88-98 | |||||
| EDTA plasma (n=4) | 95 | 89-102 | |||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Citations
- JH Cho,Animal Nutrition,2021
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