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Ganetespib [888216-25-9]

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Référence NB-64-12349-1mL

Conditionnement : 1mLx10mM(inDMSO)

Marque : Neo Biotech

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Ganetespib

(Synonyms: STA-9090) Copy Product Info

Synonyms: STA-9090

Catalog No. T2309 Copy Product Info

Purity: 99.95%

Ganetespib (STA-9090) is a synthetic small-molecule inhibitor of heat shock protein 90 (Hsp90), exhibiting potential antineoplastic activity.

Cas No. 888216-25-9

With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.95%

Color:White

Product Introduction

Bioactivity

Description	Ganetespib (STA-9090) is a synthetic small-molecule inhibitor of heat shock protein 90 (Hsp90), exhibiting potential antineoplastic activity.
Targets & IC50	HSP90:4 nM
In vitro	Ganetespib was able to exhibit cytotoxicity at the nanomolar level, inhibiting cell proliferation while inducing apoptosis in a variety of human anti-receptor tyrosine kinase inhibitor and tapepermycin-resistant cancer cell lines. In MG63 cell line (IC50=43 nM), Ganetespib inhibited cell growth. In C2 (IC50=19 nM) and BR canine malignant mast cells (IC50=4 nM), Ganetespib induced apoptosis.
In vivo	Ganetespib was able to exhibit cytotoxicity at the nanomolar level, inhibiting cell proliferation while inducing apoptosis in a variety of human anti-receptor tyrosine kinase inhibitor and tapepermycin-resistant cancer cell lines. In MG63 cell line (IC50=43 nM), Ganetespib inhibited cell growth. In C2 (IC50=19 nM) and BR canine malignant mast cells (IC50=4 nM), Ganetespib induced apoptosis.
Synonyms	STA-9090
Kinase Assay	Exponentially growing cells are processed in lysis buffer (20 mM HEPES, pH 7.4, 1 mM EDTA, 5 mM MgCl2, 100 mM KCl) and incubated with increasing concentrations of 17-AAG or ganetespib for 30 min at 4°C, and incubated with biotin-GM linked to Dynabeads MyOne Streptavidin T1 magnetic beads for 1 h at 4°C. Beads are washed three times in lysis buffer and heated for 5 min at 95°C in SDS-PAGE sample buffer. Samples are resolved on 4-12% Bis-Tris gradient gel and Western blots are performed using an anti-HSP90 antibody.
Cell Research	A total of 1.5 × 103 OSA cells are seeded in 96-well plates in 10% serum-containing complete medium and incubated overnight to determine the 50% inhibitory concentrations. Plates are, harvested at day 5 following 0.001, 0.005, 0.01, 0.05, 0.1, 0.5 and 1 μM Ganetespib, treatment and analyzed. Fluorescence measurements are made using a plate reader with excitation at 485 nm and emission detection at 530 nm. Relative cell number is calculated as a percentage of the control wells: absorbance of sample/absorbance of DMSO treated cells × 100.(Only for Reference)

Chemical Properties

Molecular Weight	364.4
Formula	C₂₀H₂₀N₄O₃
Cas No.	888216-25-9
Smiles	CC(C)c1cc(-c2n[nH]c(=O)n2-c2ccc3n(C)ccc3c2)c(O)cc1O
Relative Density.	1.39 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 83.33 mg/mL (228.68 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.49 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7442 mL	13.7212 mL	27.4424 mL	137.2119 mL
5 mM	0.5488 mL	2.7442 mL	5.4885 mL	27.4424 mL
10 mM	0.2744 mL	1.3721 mL	2.7442 mL	13.7212 mL
20 mM	0.1372 mL	0.6861 mL	1.3721 mL	6.8606 mL
50 mM	0.0549 mL	0.2744 mL	0.5488 mL	2.7442 mL
100 mM	0.0274 mL	0.1372 mL	0.2744 mL	1.3721 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

STA9090STA 9090InhibitorinhibitHSP90HSPHIF-1αHeat shock proteinsGanetespibApoptosis

Related Tags: Ganetespib chemical structure | Ganetespib in vivo | Ganetespib in vitro | Ganetespib formula | Ganetespib molecular weight