GSL II is an affinity-purified tetramer composed of a single 30 kDa subunit. Notably, it possesses insecticidal structure/function and is the first GlcNAc-binding legume lectin demonstrated to have insecticidal activity. This lectin exhibits carbohydrate specificity for α-Gal and α-GalNAc, specifically recognizing terminal GlcNAc. It elutes with galactose or N-acetylgalactosamine, and increasing the number of N-acetylglucosamine residues beyond two does not enhance affinity. GSL II uniquely recognizes exclusively α- or β-linked N-acetylglucosamine residues at the nonreducing terminal oligosaccharides and shows a preference for blood group A (-SA) over blood group B (-SA).

Recommended Usage: Recommended dilutions of 0.5 – 10 μg / ml in 1XPBS pH 7.4. 5-10 mg/ml will agglutinate Tk polyagglutinable cells.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Bioconjugation, Golgi staining, Uterine blood vesels & carcinoma marker, Blotting, Cell typing. Glycobiology.

References

1.Lamb JE, Shibata S, Goldstein IJ. Purification and Characterization of Griffonia simplicifolia Leaf Lectins. Plant Physiol. 1983 Apr;71(4):879-87.
2.Hayes CE, Goldstein IJ. An alpha-D-galactosyl-binding lectin from Bandeiraea simplicifolia seeds. Isolation by affinity chromatography and characterization. J Biol Chem. 1974 Mar 25;249(6):1904-14.
3.Delmotte FM, Goldstein IJ. Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography. Eur J Biochem. 1980 Nov;112(2):219-23.