Growth Hormone Releasing Hormone (GHRH) Recombinant, Mouse

Recombinant protein corresponding to
aa20--97 of mouse GHRH with a N-terminal His-tag expressed in E. coli. Swiss/UniProt Accession: P16043

Predicted isoelectric point:
10.5

Predicted Molecular Weight:
10.7kD

Accurate Molecular Mass:
16kD as determined by SDS-PAGE reducing conditions

Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene
2. Relative charge: The composition of amino acids may affect the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization, etc.

Subcellular Location:
Secreted

Amino Acid Sequence:
L PPSPPFRMQR HVDAIFTTNY RKLLSQLYAR KVIQDIMNKQ GERIQEQRAR LSRQEDSMWT EDKQMTLESI LQGFPRMKPS ADA

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.