Hamster interferon, gamma (IFNG) ELISA kit
Référence CSB-EL011050HA-5x96T
Conditionnement : 5x96T
Marque : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Associated names
IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
Abbreviation
Uniprot No.
Species
Mesocricetus auratus (Golden hamster)
Sample Types
serum, plasma, tissue homogenate
Detection Range
1.87 pg/mL-120 pg/mL
Sensitivity
0.46 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IFNG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
Recovery
| The recovery of hamster IFNG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
Interferon gamma (IFN-gamma) is a key cytokine produced primarily by activated T helper 1 cells, cytotoxic T lymphocytes, and natural killer cells. This pleiotropic molecule plays important roles in immune regulation, including macrophage activation, enhancement of antigen presentation, promotion of Th1 cell differentiation, and coordination of cellular immune responses against intracellular pathogens and tumors. IFN-gamma also shows antiviral properties and influences various physiological processes including cell growth regulation and apoptosis, making it a valuable biomarker in...
Citations
- HM Ahn, BK Jung, JW Hong, D Hong, A Yoon,Molecular Medicine,2025
- X Liu,Advanced science,2023
- BK Jung,J Immunother Cancer,2020
Customer Reviews and Q&A
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Target Background
Function
(From Uniprot)
(From Uniprot)
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are...
Subcellular Location
Secreted.
Protein Families
Type II (or gamma) interferon family
Tissue Specificity
Released primarily from activated T lymphocytes.