- Specifications
Product Description
Mouse monoclonal antibody raised against synthetic peptide of HDEL.
Immunogen
A synthetic peptide corresponding to C-terminus of yeast Bip HDEL.
Host
Mouse
Reactivity
Fruit fly, Plants, Yeast
Specificity
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
Form
Liquid
Isotype
IgG2B
Recommend Usage
Western Blot (1:100-1:1000)
Immunofluorescence (1:50-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunofluorescence
Western Blot
- Publication Reference
- GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development.
Luo S, Mao C, Lee B, Lee AS.
Molecular Biology of the Cell 2006 Dec; 24(12):3387.
Application:WB, Mouse, MEFs, Mouse embryos, livers.
- Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum.
Napier RM, Fowke LC, Hawes C, Lewis M, Pelham HR.
Journal of Cell Science 1992 Jun; 102 ( Pt 2):261.
Application:IEM, IF, WB-Ce, Plant, Plant cells.
- GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development.
HDEL monoclonal antibody, clone 2E7
Référence MAB7908
Conditionnement : 100ug

