Horse Leptin(LEP) ELISA kit
Référence CSB-EL012870HO-96T
Conditionnement : 96T
Marque : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
leptin
Associated names
LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit; Fragment ELISA Kit
Abbreviation
Uniprot No.
Species
Equus caballus (Horse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of horse LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 98-109 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 91-101 | |||||
Recovery
| The recovery of horse LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 86-100 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Horse LEP ELISA Kit was designed for the quantitative measurement of Horse LEP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
Citations
- SN Chewning,Thesis & Dissertation,2026
- Girardi F M, et al,Journal of Equine Veterinary Science,2019
Customer Reviews and Q&A
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Target Background
Function
(From Uniprot)
(From Uniprot)
Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism,...
Subcellular Location
Secreted.
Protein Families
Leptin family
Database Links
STRING:
UniGene: